http://www.wendyouriel.com/index daily 1.0 2017-06-01 https://images.squarespace-cdn.com/content/v1/54869789e4b00c33d16e02a1/1495526642314-P395LWODAQEROF3RNKC0/Picture6.png Scientific Writing and Publications https://images.squarespace-cdn.com/content/v1/54869789e4b00c33d16e02a1/1423037982397-BJOQKCXUG3T2OT8GZ560/Telomere_caps.gif Scientific Writing and Publications https://images.squarespace-cdn.com/content/v1/54869789e4b00c33d16e02a1/1423038173750-EIJ3PZCKCOJXWGGJIQVG/PurkinjeCell.jpg Scientific Writing and Publications https://images.squarespace-cdn.com/content/v1/54869789e4b00c33d16e02a1/1423038445054-8BZAPKXPKVQZMNGVDBCM/640px-S_cerevisiae_under_DIC_microscopy.jpg Scientific Writing and Publications https://images.squarespace-cdn.com/content/v1/54869789e4b00c33d16e02a1/1439690972538-TLVDKAO38TXLMVM14AAZ/slug-380033_1280.jpg Scientific Writing and Publications https://images.squarespace-cdn.com/content/v1/54869789e4b00c33d16e02a1/1463595194084-HA48MLUF3PXDKENI88JX/cover.gif Scientific Writing and Publications https://images.squarespace-cdn.com/content/v1/54869789e4b00c33d16e02a1/1495524686237-OLPZNU2HLONCVWAFNCOX/Picture1.jpg Scientific Writing and Publications https://images.squarespace-cdn.com/content/v1/54869789e4b00c33d16e02a1/1495525121754-GIUIIBIYBKP9Y32DZRYI/image-asset.png Scientific Writing and Publications https://images.squarespace-cdn.com/content/v1/54869789e4b00c33d16e02a1/1495525322336-G1DNNC37RSMD62Q12JTW/image-asset.png Scientific Writing and Publications Figure 2. Light micrographs of L. hesperus oocytes from unmated and mated spiders in pre-vitellogenic, vitellogenic, and post-vitellogenic phases of oocyte development. All specimens fixed using spider fixative. 2a: Oocytes in the pre-vitellogenic phase. Oocytes exhibited homogenous staining and large nuclei relative to the oocyte’s size. 2b: Early vitellogenic oocytes exhibited spherical yolk granules scattered throughout the ooplasm. A thin basal lamina was also visible at the early vitellogenic stage. 2c: Vitellogenic oocyte from an unmated spider anchored to the ovarian wall by pedicel attachment. 2d: Post-vitellogenic oocyte from a mated spider. The ooplasm was filled with yolk granules. The oocyte more than doubled in size. BL = basal lamina, G = yolk granule, LY = lipid yolk, N = nucleus, O1 = pre-vitellogenic oocyte, O2 = vitellogenic oocyte, O3 = post-vitellogenic oocyte, OL = ovarian lumen, OT = ovarian tissue, P =pedicel, PY = protein yolk     https://images.squarespace-cdn.com/content/v1/54869789e4b00c33d16e02a1/1495525426857-QGP2KQNOHSX1056Z6PC0/image-asset.png Scientific Writing and Publications Figure 3. Transmission electron micrographs of unmated L. hesperus oocytes in pre-vitellogenic and vitellogenic phases. Dissected ovarian tissue. 3a: Pre-vitellogenic (Phase 1) oocyte. The ooplasm has a granular appearance. A thin basal lamina was observed surrounding the oocyte and pedicel. 3b: Pre-vitellogenic (Stage 1) oocyte sectioned through the nucleus. Mitochondria were observed in clusters around the nuclear envelope 3c: Early vitellogenic (Stage 2) oocyte. Lipid accumulation was seen as electron-dense granules throughout the ooplasm. There is a narrow space between the basal lamina and plasma membrane. The ooplasm is dense with lipid and protein yolk granules. The oocyte had increased in size. 3d: Late-vitellogenic oocyte. Numerous large lipid and protein yolk granules were in the ooplasm. BL = basal lamina, G = granules, LY= lipid yolk granule, N = nucleus, O2 = vitellogenic oocyte, O2E = early vitellogenic oocyte, O2L = late vitellogenic oocyte P = pedicel attachment, PM = plasma membrane, PY = protein yolk granule, Y = yolk granules https://images.squarespace-cdn.com/content/v1/54869789e4b00c33d16e02a1/1495525558084-Z9E3I8T5X67UDR0DIP94/image-asset.png Scientific Writing and Publications Figure 4. Changes in the basal lamina and plasma membrane between the pre-vitellogenic and late vitellogenic phases/stages of unmated L. hesperus oocyte development. Whole abdomens fixed using spider fixative. 4a: Light micrograph field of view containing oocytes in the pre-vitellogenic, early vitellogenic, and late vitellogenic phases. Area in box is shown at higher magnification below 4b: Electron micrograph of the pre-vitellogenic oocyte pictured in 4a (solid line). 4c: Electron micrograph of the late vitellogenic oocyte pictured in 4a (dotted line box). The basal lamina had thickened from 0.161 μm (4b) to 0.175 μm. The space between the basal lamina and plasma membrane had also widened to 2.5 μm. Note the presence of clathrin-coated pits. BL = basal lamina, CP = coated pits, CV = coated vesicle, VL = Vitelline membrane, LY = lipid yolk, MV = microvilli, O2E = early vitellogenic oocyte, O2L = late vitellogenic oocyte, PV = pre-vitellogenic oocyte, PY = protein yolk. https://images.squarespace-cdn.com/content/v1/54869789e4b00c33d16e02a1/1495525809981-E1SXVAP4R3I05K8ZIFC2/image-asset.png Scientific Writing and Publications Figure 6. Whole abdomen section of L. hesperus at the beginning of oviposition. Whole abdomen prepared using Bouin’s fixative. The post-vitellogenic oocytes (ova) measured 1.08 ± 0.58 mm3 in volume (n = 7). Ova and pre-vitellogenic oocytes are separate from each other. The post-vitellogenic oocytes are contained within their own membrane-bound compartment. Pedicels were seen attached to the membrane bound compartment (arrowhead). Note that no vitellogenic oocytes are visible. H = heart, MA = major ampullate silk gland, O1 = pre-vitellogenic oocyte, O3 = post-vitellogenic oocyte, T = tubuliform silk gland. https://images.squarespace-cdn.com/content/v1/54869789e4b00c33d16e02a1/1495525904701-WVO9U5HAMK0SFGGLMTJ2/image-asset.png Scientific Writing and Publications Figure 7. Light micrographs of whole body section of L. hesperus ovarian tissue acquired during late oviposition. Whole abdomen fixed using Bouin's fixative. 7a: Multiple oocytes in pre-vitellogenic phase of development were observed. There was only one vitellogenic, two post-vitellogenic oocytes observed during late oviposition. Post-vitellogenic oocytes depicted were not spherical, which may be an artifact or due to the plane of sectioning. 7b: Increased magnification of the specimen is shown in 7a (boxed selection). The space between the mature ova and pre-vitellogenic oocytes was more apparent. He = hemocoel, O1=pre-vitellogenic oocyte, O2 = vitellogenic oocyte O3 = post‑vitellogenic oocyte, OW = ovarian wall. https://images.squarespace-cdn.com/content/v1/54869789e4b00c33d16e02a1/1495525992011-81EL7N8ZW9VUKLYKOVJY/image-asset.png Scientific Writing and Publications Figure 8. Light micrographs of whole abdominal sections of L. hesperus approximately five hours after oviposition. Whole abdomen fixed using spider fixative. 8a: Sections taken about 1.4 mm deep into abdomen (diagram shown). One end of the major ampullate gland was visible in the section. Only pre-vitellogenic oocytes were visible in this post-oviposition spider. Note the empty space (**). 8b: Specimen depicted in Figure 8a, sectioned 450 μm deeper into the abdomen. The section cut directly through the major ampullate gland. Note that the ovarian tissue enclosed the space where the pre-vitellogenic oocytes were found (asterisk). Oocytes are observed on both sides of the enclosed space (**). 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